Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| hyaluronoglucosaminidase 1 | Polyclonal | IgG | Rabbit | Human | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human HYAL1(32-47aa FTTVWNANTQWCLERH). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HYAL1 |
Protein |
Hyaluronidase-1 |
Uniprot ID |
Q12794 |
Function |
May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. |
Tissue Specificity |
Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the glycosyl hydrolase 56 family. |
Aliases |
HYAL 1 antibody|Hyal-1 antibody|HYAL1 antibody|HYAL1_HUMAN antibody|Hyaluronidase 1 antibody|Hyaluronidase-1 antibody|Hyaluronoglucosaminidase 1 antibody|Hyaluronoglucosaminidase-1 antibody|Hyaluronoglucosaminidase1 antibody|LUCA 1 antibody|LUCA-1 antibody|LUCA1 antibody|Lung carcinoma protein 1 antibody|MGC45987 antibody|NAT6 antibody|Plasma hyaluronidase antibody|Tumor suppressor LUCA 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-HYAL1 antibody, ASA-B0953, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-HYAL1 antibody, ASA-B0953, ICC
ICC: MCF-7 Cell
ICC: MCF-7 Cell
Anti-HYAL1 antibody, ASA-B0953, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: 22RV1 Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: 22RV1 Cell Lysate
Lane 3: MCF-7 Cell Lysate
