Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| hydroxysteroid(17-beta) dehydrogenase 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Hydroxysteroid(17-beta) Dehydrogenase 4(744-761aa NIMLSQKLQMILKDYAKL), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HSD17B4 |
Protein |
Enoyl-CoA hydratase 2 |
Uniprot ID |
F5HE57 |
Function |
Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. |
Tissue Specificity |
Present in many tissues with highest concentrations in liver, heart, prostate and testis. |
Sub-cellular localization |
Peroxisome. |
Sequence Similarities |
Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
Aliases |
12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody|17 beta HSD 4 antibody|17 beta HSD IV antibody|17 beta hydroxysteroid dehydrogenase 4 antibody|17-beta-HSD 4 antibody|17-beta-hydroxysteroid dehydrogenase 4 antibody|17beta estradiol dehydrogenase type IV antibody|3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase antibody|3-alpha antibody|7-alpha antibody|Beta hydroxyacyl dehydrogenase antibody|Beta keto reductase antibody|D 3 hydroxyacyl CoA dehydratase antibody|D bifunctional protein antibody|D bifunctional protein peroxisomal antibody|D-bifunctional protein antibody|DBP antibody|DHB4_HUMAN antibody|EDH17B4 antibody|Enoyl-CoA hydratase 2 antibody|Hsd17b4 antibody|MFE 2 antibody|MFE-2 antibody|MPF-2 antibody|Multifunctional protein 2 antibody|Peroxisomal multifunctional enzyme type 2 antibody|Peroxisomal multifunctional protein 2 antibody|SDR8C1 antibody|Short chain dehydrogenase/reductase family 8C member 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
-Dehydrogenase-4-antibody-ASA-B0957-1.jpg)
Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 antibody, ASA-B0957, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Small Intestine Tissue Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: U87 Cell Lysate
Lane 8: COLO320 Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Small Intestine Tissue Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: U87 Cell Lysate
Lane 8: COLO320 Cell Lysate
-Dehydrogenase-4-antibody-ASA-B0957-2.jpg)
Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 antibody, ASA-B0957, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
-Dehydrogenase-4-antibody-ASA-B0957-3.jpg)
Anti-Hydroxysteroid(17-beta) Dehydrogenase 4 antibody, ASA-B0957, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
