Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| indian hedgehog | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of human Ihh(250-264aa EPHRLRAFQVIETQD), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IHH |
Protein |
Indian hedgehog protein |
Uniprot ID |
Q14623 |
Function |
Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP) (By similarity). |
Tissue Specificity |
Expressed in embryonic lung, and in adult kidney and liver. |
Sub-cellular localization |
Indian hedgehog protein N-product: Cell membrane ; Lipid-anchor ; Extracellular side . Note: The N-terminal peptide remains associated with the cell surface. |
Sequence Similarities |
|
Aliases |
BDA1 antibody|HHG-2 antibody|HHG2 antibody|IHH antibody|IHH_HUMAN antibody|Indian Hedgehog antibody|Indian hedgehog homolog antibody|Indian hedgehog protein antibody|Indian hedgehog protein C-product antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Ihh antibody, ASA-B0978, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: HEPG2 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: HEPG2 Cell Lysate
