Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human IKB beta recombinant protein (Position: E56-E237). Human IKB beta shares 82% and 80% amino acid (aa) sequence identity with mouse and rat IKB beta, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NFKBIB |
Protein |
NF-kappa-B inhibitor beta |
Uniprot ID |
Q15653 |
Function |
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA- dependent inactivation. Association with inhibitor kappa B- interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation. |
Tissue Specificity |
Expressed in all tissues examined. |
Sub-cellular localization |
Cytoplasm . Nucleus . |
Sequence Similarities |
Belongs to the NF-kappa-B inhibitor family. |
Aliases |
I kappa B beta antibody|I-kappa-B-beta antibody|IkappaBbeta antibody|IKB beta antibody|IkB-B antibody|IkB-beta antibody|IKBB antibody|IKBB_HUMAN antibody|IkBbeta antibody|NF kappa BIB antibody|NF-kappa-B inhibitor beta antibody|NF-kappa-BIB antibody|Nfkbib antibody|Thyroid receptor interacting protein 9 antibody|Thyroid receptor-interacting protein 9 antibody|TR interacting protein 9 antibody|TR-interacting protein 9 antibody|TRIP-9 antibody|TRIP9 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IKB beta antibody, ASA-B0981, Western blotting
All lanes: Anti IKB beta (ASA-B0981) at 0.5ug/ml
Lane 1: Mouse Kidney Tissue Lysate at 50ug
Lane 2: RH35 Whole Cell Lysate at 40ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti IKB beta (ASA-B0981) at 0.5ug/ml
Lane 1: Mouse Kidney Tissue Lysate at 50ug
Lane 2: RH35 Whole Cell Lysate at 40ug
Lane 3: NRK Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD
Anti- IKB beta antibody, ASA-B0981,IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti- IKB beta antibody, ASA-B0981,IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
