Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| interleukin 18 | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived mouse IL18 recombinant protein (Position: N36-S192). Mouse IL18 shares 66% and 91% amino acid (aa) sequence identity with human and rat IL18, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IL18 |
Protein |
Interleukin-18 |
Uniprot ID |
P70380 |
Function |
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
Iboctadekin antibody|IFN gamma inducing factor antibody|IFN-gamma-inducing factor antibody|IGIF antibody|IL 1 gamma antibody|IL 18 antibody|IL 1g antibody|IL-1 gamma antibody|IL-18 antibody|IL1 gamma antibody|IL18 antibody|IL18 protein antibody|IL18_HUMAN antibody|IL1F4 antibody|IL1g antibody|IL1gamma antibody|ILIF4 antibody|Interferon gamma inducing factor antibody|Interferon gamma-inducing factor antibody|Interleukin 1 gamma antibody|Interleukin 18 antibody|Interleukin-1 gamma antibody|Interleukin-18 antibody|Interleukin18 antibody|MGC12320 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IL18 antibody, ASA-B1011, Western blotting
All lanes: Anti IL18 (ASA-B1011) at 0.5ug/ml
WB: Recombinant Mouse IL18 Protein 0.5ng
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti IL18 (ASA-B1011) at 0.5ug/ml
WB: Recombinant Mouse IL18 Protein 0.5ng
Predicted bind size: 22KD
Observed bind size: 22KD
Anti- IL18 antibody, ASA-B1011, Western blotting
All lanes: Anti IL18 (ASA-B1011) at 0.5ug/ml
WB: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 50KD
All lanes: Anti IL18 (ASA-B1011) at 0.5ug/ml
WB: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 22KD
Observed bind size: 50KD
