Description
Data Sheet
| Applications | Influenza A haemagglutinin H1N1 Western Blotting at a concentration of 0.5ug/ml. ELISA to be determined. |
| Clone | IHA-H1N1-PRS. |
| Description | Influenza Hemagglutinin protein is an envelope glycoprotein responsible for binding to sialic receptors and influenza viral entry into host cells. The antibody was produced by immunization of rabbits with purified recombinant influenza A/New Caledonia/20/99 produced in insect cells using baculovirus expression vector system. The antigen was purified under conditions that preserve the HA proteins biological activity and tertiary structure. |
| Immunogen | Recombinant Influenza A/New Caledonia/20/99. |
| Inactivation | Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| Protein Background | H1N1 is a subtype specie of Influenza A virus. H1N1 Influenza Virus has mutated into various strains such as the Spanish Flu strain, mild human flu strains, endemic pig strains, and various strains found in birds. The Influenza A Virus is a globular particle about 100nm in diameter, sheathed in a lipid bilayer derived from the plasma membrane of its host. Studded in the lipid bilayer are two integral membrane proteins some 500 molecules of hemagglutinin (“H”) and some 100 molecules of neuraminidase (“N”). Within the lipid bilayer are 3000 molecules of matrix protein and 8 pieces of RNA. Each of the 8 RNA molecules is associated with many copies of a nucleoprotein, several molecules of the three subunits of its RNA polymerase some “non-structural” protein molecules of uncertain function. |
| Purity | 90%. |
| Storage Procedures | Store at -20°C. |
| Type | Rabbit Antibody Polyclonal. |
