Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| integrin, alpha V | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin alpha V(1028-1048aa QEEQEREQLQPHENGEGNSET), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ITGAV |
Protein |
Integrin alpha-V |
Uniprot ID |
P06756 |
Function |
The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi’s sarcoma lesions. |
Tissue Specificity |
|
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Belongs to the integrin alpha chain family. |
Aliases |
antigen identified by monoclonal antibody L230 antibody|CD 51 antibody|CD51 antibody|CD51 antigen antibody|DKFZp686A08142 antibody|HGNC antibody|integrin alpha V beta 3 antibody|integrin alpha-V antibody|Integrin alpha-V light chain antibody|integrin alphaVbeta3 antibody|integrin, alpha V(vitronectin receptor, alpha polypeptide, antigen CD51) antibody|ITAV_HUMAN antibody|ITGA V antibody|ITGAV antibody|Msk 8 antibody|Msk8 antibody|Vitronectin receptor alpha polypeptide antibody|Vitronectin receptor alpha polypeptide antigen CD51 antibody|Vitronectin receptor subunit alpha antibody|VNRA antibody|VTNR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Integrin alpha V antibody, ASA-B1047, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: MM231 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: CEM Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: MM231 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: CEM Cell Lysate
Lane 5: COLO320 Cell Lysate
Anti-Integrin alpha V antibody, ASA-B1047, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Integrin alpha V antibody, ASA-B1047, ICC
ICC: A549 Cell
ICC: A549 Cell
