Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| eukaryotic translation initiation factor 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human integrin beta 4 binding protein(82-96aa QHIRNSLPDTVQIRR), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
EIF6 |
Protein |
Eukaryotic translation initiation factor 6 |
Uniprot ID |
P56537 |
Function |
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. |
Tissue Specificity |
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). |
Sub-cellular localization |
Cytoplasm. Nucleus, nucleolus. Note: Shuttles between cytoplasm and nucleus/nucleolus. |
Sequence Similarities |
|
Aliases |
b(2)gcn antibody|B(2)GCN homolog antibody|B4 integrin interactor antibody|Binding protein of beta-4 integrin antibody|CAB antibody|eIF-6 antibody|EIF3A antibody|EIF6 antibody|Eukaryotic translation initiation factor 3A antibody|Eukaryotic translation initiation factor 6 antibody|IF6_HUMAN antibody|ITGB4BP antibody|OK/SW-cl.27 antibody|p27 beta 4 integrin binding protein antibody|p27(BBP) antibody|p27BBP antibody|RP4-614O4.1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Mouse Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-integrin beta 4 binding protein antibody, ASA-B1052, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: COLO320 Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: HEPA Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: COLO320 Cell Lysate
Lane 4: SW620 Cell Lysate
Lane 5: HELA Cell Lysate
Lane 6: 293T Cell Lysate
Lane 7: HEPA Cell Lysate
Anti-integrin beta 4 binding protein antibody, ASA-B1052, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-integrin beta 4 binding protein antibody, ASA-B1052, IHC(F)
IHC(F): Human Placenta Tissue
IHC(F): Human Placenta Tissue
