| Amino acid sequence |
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N. |
| Biological Activity |
The ED50 as determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells was found to be less than 2.0ng/ml, corresponding to a specific activity of 5.0105 IU/mg. |
| Description |
Interleukin-10 Human Recombinant produced in E.coli is a single non-glycosylated polypeptide chains containing 161 amino acids each and having a molecular mass of 18.6kDa.The IL-10 is purified by proprietary chromatographic techniques. |
| Expression host |
Escherichia Coli. |
| Formulation |
Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Protein Background |
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
| Purity |
Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| References |
1.Title:Activated signal transducer and activator of transcription-3 (STAT3) is a poor regulator of tumour necrosis factor- production by human monocytes.Publication:Article first published online: 9 JAN 2007 DOI:10.1111/j.1365-2249.2006.03291.xLink:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2006.03291.x/full2.Title:Suppressor of cytokine signalling-3 at pathological levels does not regulate lipopolysaccharide or interleukin-10 control of tumour necrosis factor-? production by human monocytes.Publication:Article first published online: 11 MAY 2006 DOI: 10.1111/j.1365-2567.2006.02383.xLink:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2567.2006.02383.x/full3.Title:The Tyrosine Sulfate-rich Domains of the LRR Proteins Fibromodulin and Osteoadherin Bind Motifs of Basic Clusters in a Variety of Heparin-binding Proteins, Including Bioactive Factors*.Publication:First Published on August 21, 2009, doi: 10.1074/jbc.M109.047076 October 16, 2009 The Journal of Biological Chemistry, 284, 28543-28553.Link:http://www.jbc.org/content/284/42/28543.full |
| Solubility |
It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms |
B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor. |
| Titer |
Antibody is shipped in liquid form with ice packs. |
