Description
Data Sheet
| Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Pro-Pro-Gly. N-terminal methionine has been completely removed enzymatically. |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of murine 7TD1 was found to be |
| Description | Interleukin-11 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19256.29 Dalton. The IL-11 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with no additives. |
| Immunological Activity | Thimerosal and beta propiolactone treatmentThis product has been treated in a manner consistent with methods of inactivation. Generally accepted good laboratory practices appropriate to microbiological/viral safe handling practices and techniques are required at work. |
| Protein Background | IL11 is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). IL-11 is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Reference | Tested with anti-influenza B monoclonal antibodies in ELISA.Serological studies of influenza B virus, immunogen for antibody production. |
| References | Title: Models for Prediction of Factor VIII Half-Life in Severe Haemophiliacs: Distinct Approaches for Blood Group O and Non-O Patients Publication: PloS one 4.8 (2009): e6745. Link: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0006745 |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin 11 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Interleukin-11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL11 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | AGIF, Adipogenesis inhibitory factor, Oprelvekin, IL-11. |
