Description
Aldesleukin, IL-2, Lymphokine, T-cell growth factor (TCGF)
Data Sheet
| Amino acid sequence | MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT. |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is |
| Description | Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15kDa. The IL-2 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein (1mg/ml) was lyophilized after extensive dialysis against 20mM PBS pH=7.4. |
| Protein Background | IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. |
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | 1.Title:CD8 Kinetically Promotes Ligand Binding to the T-Cell Antigen Receptor.Publication:Biophysical Journal Volume 89 September 2005 21212133.Link:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1366714/pdf/2121.pdf2.Title: Detection of expressed IL-32 in human stomach cancer using ELISA and immunostainingPublication: J Microbiol Biotechnol 18.9 (2008): 1606-1612.Link: http://www.jmb.or.kr/submission/Journal/018/JMB018-09-19.pdf3. Title: Conservation of cytokine-mediated responses in innate immunity: a flow cytometric study investigating the effects of human proinflammatory cytokines on phagocytosis in the earthworm Eisenia hortensisPublication: Invertebrate Survival Journal 5 (2008): 124-134.Link: http://www.isj.unimo.it/articoli/ISJ175.pdf |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin 2 in 20mM AcOH not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2. |
