Description
Data Sheet
| Amino acid sequence | ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI. |
| Biological Activity | The ED50was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0106 IU/mg. |
| Description | Interleukin-7 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14.9kDa. |
| Expression host | Escherichia Coli. |
| Formulation | Filtered (0.2um) and lyophilized from a concentrated (1mg/ml) solution in 1PBS, pH7.4 and 2% trehalose. |
| Protein Background | Interleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloid lineages. |
| Purity | Greater than 96.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | Title: Disruption of Bis Leads to the Deterioration of the Vascular Niche for Hematopoietic Stem CellsPublication: Stem Cells 28.2 (2010): 268-278.Link: http://onlinelibrary.wiley.com/doi/10.1002/stem.285/full |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | Lymphopoietin 1 (LP-1), pre-B cell factor, IL-7. |
