Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| glutamate receptor, ionotropic, AMPA 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Ionotropic Glutamate receptor 2(260-277aa FQIVDYDDSLVSKFIERW), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GRIA2 |
Protein |
Glutamate receptor 2(GluR-2) |
Uniprot ID |
P42262 |
Function |
Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
|
Aliases |
AMPA 2 antibody|AMPA selective glutamate receptor 2 antibody|AMPA-selective glutamate receptor 2 antibody|AMPA2 antibody|Glu R2 antibody|Glu RB antibody|GluA2 antibody|GluR 2 antibody|GluR B antibody|GluR B flop antibody|GluR K2 antibody|GluR-2 antibody|GluR-B antibody|GluR-K2 antibody|GLUR2 antibody|GluRB antibody|Glutamate receptor 2 antibody|Glutamate receptor ionotropic AMPA 2 antibody|Glutamate receptor ionotropic AMPA2 antibody|Glutamate receptor ionotropic antibody|Gria 2 antibody|Gria2 antibody|GRIA2_HUMAN antibody|HBGR 2 antibody|HBGR2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Ionotropic Glutamate receptor 2 antibody, ASA-B1057, Western blotting
All lanes: Anti Ionotropic Glutamate receptor 2 (ASA-B1057) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
All lanes: Anti Ionotropic Glutamate receptor 2 (ASA-B1057) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
Anti-Ionotropic Glutamate receptor 2 antibody, ASA-B1057, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
