Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| interleukin-1 receptor-associated kinase 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human IRAK2(600-616aa EAKRKLMENILLYKEEK), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IRAK2 |
Protein |
Interleukin-1 receptor-associated kinase-like 2 |
Uniprot ID |
O43187 |
Function |
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. |
Tissue Specificity |
Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. |
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. |
Aliases |
IRAK-2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-IRAK2 antibody, ASA-B1062, Western blotting
Lane 1: 22RV Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 1: 22RV Cell Lysate
Lane 2: A549 Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: SMMC Cell Lysate
Anti-IRAK2 antibody, ASA-B1062, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
Anti-IRAK2 antibody, ASA-B1062, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue
