Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| insulin receptor substrate 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human IRS2 recombinant protein (Position: D16-V200). Human IRS2 shares 88% amino acid (aa) sequence identity with mouse IRS2. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
IRS2 |
Protein |
Insulin receptor substrate 2 |
Uniprot ID |
Q9Y4H2 |
Function |
May mediate the control of various cellular processes by insulin. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm, cytosol . |
Sequence Similarities |
Contains 1 IRS-type PTB domain. |
Aliases |
Insulin receptor substrate 2 antibody|IRS 2 antibody|IRS-2 antibody|Irs2 antibody|IRS2_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- IRS2 antibody, ASA-B1075, Western blotting
All lanes: Anti IRS2 (ASA-B1075) at 0.5ug/ml
WB: Recombinant Human IRS2 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti IRS2 (ASA-B1075) at 0.5ug/ml
WB: Recombinant Human IRS2 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- IRS2 antibody, ASA-B1075, Western blotting
All lanes: Anti IRS2 (ASA-B1075) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 137KD
Observed bind size: 137KD
All lanes: Anti IRS2 (ASA-B1075) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MM231 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 137KD
Observed bind size: 137KD
