Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| jagged 2 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Jagged 2 recombinant protein (Position: R962-E1238). Human Jagged 2 shares 85% and 79% amino acid (aa) sequence identity with mouse and rat Jagged 2, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
JAG2 |
Protein |
Protein jagged-2 |
Uniprot ID |
Q9Y219 |
Function |
Putative Notch ligand involved in the mediation of Notch signaling. Involved in limb development (By similarity). |
Tissue Specificity |
Expressed in heart, placenta and skeletal muscle and to a lesser extend in pancreas. Very low expression in brain, lung, liver and kidney. |
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Contains 1 DSL domain. |
Aliases |
D12Ggc2e antibody|HJ 2 antibody|HJ2 antibody|JAG 2 antibody|JAG2 antibody|Jagged 2 precursor antibody|Jagged2 antibody|Protein jagged 2 antibody|SER2 antibody|Syndactylism antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- antibody, PB, Western blotting
All lanes: Anti Jagged 2 (ASA-B1083) at 0.5ug/ml
WB: Recombinant Human Jagged 2 Protein 0.5ng
Predicted bind size: 39 KD
Observed bind size: 39 KD
All lanes: Anti Jagged 2 (ASA-B1083) at 0.5ug/ml
WB: Recombinant Human Jagged 2 Protein 0.5ng
Predicted bind size: 39 KD
Observed bind size: 39 KD
Anti- antibody, PB, Western blotting
All lanes: Anti Jagged 2 (ASA-B1083) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 134KD
Observed bind size: 134KD
All lanes: Anti Jagged 2 (ASA-B1083) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 134KD
Observed bind size: 134KD
