Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| jun B proto-oncogene | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human JunB (323-347aa QLKQKVMTHVSNGCQLLLGVKGHAF), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
JUNB |
Protein |
Transcription factor jun-B |
Uniprot ID |
P17275 |
Function |
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5′-TGA[CG]TCA-3′. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the bZIP family. Jun subfamily. |
Aliases |
Activator protein 1 antibody|AP 1 antibody|AP1 antibody|Jun B antibody|Jun B proto oncogene antibody|Jun B protooncogene antibody|Junb antibody|JunB proto oncogene antibody|JunB protoncogene 9 antibody|JunB protooncogene antibody|JUNB_HUMAN antibody|Transcription factor jun B antibody|Transcription factor jun-B antibody|Transcription factor junB antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-JunB antibody, ASA-B1088, Western blotting
All lanes: Anti JunB (ASA-B1088) at 0.5ug/ml
WB: Recombinant Human JunB Protein 0.5ng
Predicted bind size: 19KD
Observed bind size: 19KD
All lanes: Anti JunB (ASA-B1088) at 0.5ug/ml
WB: Recombinant Human JunB Protein 0.5ng
Predicted bind size: 19KD
Observed bind size: 19KD
Anti-JunB antibody, ASA-B1088, Western blotting
All lanes: Anti JunB (ASA-B1088) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti JunB (ASA-B1088) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD
Anti-JunB antibody, ASA-B1088, IHC(P)
IHC(P): Mouse Brain Tissue
IHC(P): Mouse Brain Tissue
