Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| kallikrein-related peptidase 2 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 2(243-261aa YTKVVHYRKWIKDTIAANP). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
KLK2 |
Protein |
Kallikrein-2 |
Uniprot ID |
P20151 |
Function |
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. |
Tissue Specificity |
|
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the peptidase S1 family. Kallikrein subfamily. |
Aliases |
Glandular kallikrein 2 antibody|Glandular kallikrein-1 antibody|hGK 1 antibody|hGK-1 antibody|hK2 antibody|Kallikrein 2 prostatic antibody|Kallikrein related peptidase 2 antibody|kallikrein, glandular antibody|Kallikrein, prostatic antibody|Kallikrein-2 antibody|KLK 2 antibody|Klk-2 antibody|Klk2 antibody|KLK2_HUMAN antibody|KLK2A 2 antibody|KLK2A2 antibody|MGC12201 antibody|Tissue kallikrein 2 antibody|Tissue kallikrein-2 antibody|Ton antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Kallikrein 2 antibody, ASA-B1095, Western blotting
All lanes: Anti Kallikrein 2 (ASA-B1095) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 55KD
All lanes: Anti Kallikrein 2 (ASA-B1095) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 55KD
Anti-Kallikrein 2 antibody, ASA-B1095, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
