Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 4 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Kallistatin(384-401aa AIKFFSAQTNRHILRFNR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SERPINA4 |
Protein |
Kallistatin |
Uniprot ID |
P29622 |
Function |
Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. |
Tissue Specificity |
Expressed by the liver and secreted in plasma. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the serpin family. |
Aliases |
KAIN_HUMAN antibody|KAL antibody|Kallikrein binding protein antibody|Kallikrein inhibitor antibody|Kallistatin antibody|KBP antibody|KLST antibody|KST antibody|MGC108582 antibody|Peptidase inhibitor 4 antibody|PI-4 antibody|PI4 antibody|Protease inhibitor 4(kallistatin) antibody|Protease inhibitor 4 antibody|Serine(or cysteine) proteinase inhibitor, clade A(alpha 1 antiproteinase, antitrypsin), member 4 antibody|Serpin A4 antibody|Serpin peptidase inhibitor clade A member 4 antibody|Serpin peptidase inhibitor, clade A(alpha 1 antiproteinase, antitrypsin), member 4 antibody|SERPINA4 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Kallistatin antibody, ASA-B1100, IHC(P)
IHC(P): Human Liver Cancer Tissue
IHC(P): Human Liver Cancer Tissue
Anti-Kallistatin antibody, ASA-B1100, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 1: SMMC Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: JURKAT Cell Lysate
Anti-Kallistatin antibody, ASA-B1100, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SKOV Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: SKOV Cell Lysate
