Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| kelch-like ECH-associated protein 1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Keap1(46-60aa QHGNRTFSYTLEDHT), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
KEAP1 |
Protein |
Kelch-like ECH-associated protein 1 |
Uniprot ID |
Q14145 |
Function |
Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. |
Tissue Specificity |
Broadly expressed, with highest levels in skeletal muscle. |
Sub-cellular localization |
Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus. |
Sequence Similarities |
Contains 1 BACK (BTB/Kelch associated) domain. |
Aliases |
Cytosolic inhibitor of Nrf2 antibody|INrf 2 antibody|INrf2 antibody|Keap 1 antibody|KEAP1 antibody|KEAP1_HUMAN antibody|Kelch like ECH associated protein 1 antibody|Kelch like family member 19 antibody|Kelch like protein 19 antibody|Kelch-like ECH-associated protein 1 antibody|Kelch-like protein 19 antibody|KIAA0132 antibody|KLHL 19 antibody|KLHL19 antibody|MGC10630 antibody|MGC1114 antibody|MGC20887 antibody|MGC4407 antibody|MGC9454 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Keap1 antibody, ASA-B1115, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HT1080 Cell Lysate
Lane 3: PANC Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: HT1080 Cell Lysate
Lane 3: PANC Cell Lysate
