Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| Kruppel-like factor 8 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8(1-18aa MVDMDKLINNLEVQLNSE). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
KLF8 |
Protein |
Krueppel-like factor 8 |
Uniprot ID |
O95600 |
Function |
Transcriptional repressor and activator. Binds to CACCC- boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK). |
Tissue Specificity |
Ubiquitous. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the Sp1 C2H2-type zinc-finger protein family. |
Aliases |
Basic krueppel-like factor 3 antibody|BKLF3 antibody|DXS741 antibody|KLF8 antibody|KLF8_HUMAN antibody|Krueppel-like factor 8 antibody|Kruppel like factor 8 antibody|MGC138314 antibody|Zinc finger protein 741 antibody|ZNF741 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-KLF8 antibody, ASA-B1131, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: 293T Cell Lysate
Lane 1: SMMC Cell Lysate
Lane 2: 293T Cell Lysate
