Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| lamin B2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B2(580-597aa EEDLFHQQGDPRTTSRGC), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
LMNB2 |
Protein |
Lamin-B2 |
Uniprot ID |
Q03252 |
Function |
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side. |
Sequence Similarities |
Belongs to the intermediate filament family. |
Aliases |
LAMB 2 antibody|LAMB2 antibody|Lamin-B2 antibody|LMN 2 antibody|LMN B2 antibody|LMN2 antibody|LMNB 2 antibody|LMNB2 antibody|LMNB2_HUMAN antibody|MGC2721 antibody|RGD1563803 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Lamin B2 antibody, ASA-B1151, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Anti-Lamin B2 antibody, ASA-B1151, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
