Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, beta 2(laminin S) |
Monoclonal |
IgG |
Mouse |
Feline, Human, Pig |
IHC-P, WB |
Ascites |
Immunogen
|
| Human laminin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
LAMA1; LAMA2; LAMB1; LAMB2 |
Protein
|
Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2 |
Uniprot ID
|
Human(P25391), Mouse(P19137), Rat(D4A409), Human(P24043), Mouse(Q60675), Rat(F1M614), Human(P07942), Mouse(P02469), Rat(D3ZQN7), Human(P55268), Mouse(Q61292), Rat(P15800) |
Function
|
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. |
Tissue Specificity
|
|
Sub-cellular localization
|
Secreted, extracellular space, extracellular matrix, basement membrane. Note: Major component. |
Sequence Similarities
|
Contains 17 laminin EGF-like domains. |
Aliases
|
LAMA|Laminin a|LAMM|CLM|LIS5|LAMB1|LAMS|NPHS5|Laminin M chain|Laminin-12 subunit alpha|Laminin-2 subunit alpha|Laminin-4 subunit alpha|Merosin heavy chain|S-LAM beta |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.25-0.5μg/ml |
Human, feline, pig |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human, feline, pig |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
