Description
Data Sheet
| Amino acid sequence | The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Thr-Pro-ValBiological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio. |
| Description | Leptin Binding Domain Human Recombinant also called Leptin soluble receptor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 428-635 of human leptin receptor.The Leptin Binding Domain is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility | The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point. |
| Stability | Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.Please prevent freeze-thaw cycles. |
| Synonyms | OB Protein, Obesity Protein, OBS, Obesity factor, Leptin Receptor. |
