Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| dihydrolipoamide dehydrogenase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Lipoamide Dehydrogenase(492-509aa EAFREANLAASFGKSINF), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DLD |
Protein |
Dihydrolipoyl dehydrogenase, mitochondrial |
Uniprot ID |
P09622 |
Function |
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction. |
Tissue Specificity |
|
Sub-cellular localization |
Mitochondrion matrix. |
Sequence Similarities |
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. |
Aliases |
Diaphorase antibody|Dihydrolipoamide dehydrogenase antibody|Dihydrolipoyl dehydrogenase antibody|Dihydrolipoyl dehydrogenase mitochondrial antibody|dld antibody|DLDH antibody|DLDH_HUMAN antibody|E3 antibody|E3 component of pyruvate dehydrogenase antibody|E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex antibody|GCSL antibody|Glycine cleavage system L protein antibody|Glycine cleavage system protein L antibody|LAD antibody|lipoamide dehydrogenase antibody|Lipoamide reductase antibody|Lipoyl dehydrogenase antibody|mitochondrial antibody|OTTHUMP00000206744 antibody|OTTHUMP00000206746 antibody|OTTHUMP00000206748 antibody|OTTHUMP00000206749 antibody|PHE 3 antibody|PHE3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Lipoamide Dehydrogenase antibody, ASA-B1171, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: Rat Testis Tissue Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: JURKAT Cell Lysate
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: Rat Testis Tissue Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: JURKAT Cell Lysate
Anti-Lipoamide Dehydrogenase antibody, ASA-B1171, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Lipoamide Dehydrogenase antibody, ASA-B1171, ICC
ICC: MCF-7 Cell
ICC: MCF-7 Cell
