Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| O-6-methylguanine-DNA methyltransferase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human MGMT(87-101aa PVFQQESFTRQVLWK), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MGMT |
Protein |
Methylated-DNA–protein-cysteine methyltransferase |
Uniprot ID |
P16455 |
Function |
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
|
Aliases |
6 O methylguanine DNA methyltransferase antibody|Agat antibody|AGT antibody|AI267024 antibody|EC 2.1.1.63 antibody|Methylated DNA protein cysteine methyltransferase antibody|Methylguanine DNA methyltransferase antibody|MGC107020 antibody|MGMT antibody|MGMT: O-6 methylguanine DNA methyltransferase antibody|O 6 methylguanine DNA alkyltransferase antibody|O 6 methylguanine DNA methyltransferase antibody|O-6 methylguanine DNA alkyltransferase antibody|O-6-methylguanine-DNA methyltransferase antibody|O6 methylguanine DNA methyltransferase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MGMT antibody, ASA-B1269, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: Rat Thymus Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: JURKAT Cell Lysate
Lane 8: RAJI Cell Lysate
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Spleen Tissue Lysate
Lane 4: Rat Thymus Tissue Lysate
Lane 5: MCF-7 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: JURKAT Cell Lysate
Lane 8: RAJI Cell Lysate
