Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| pyruvate dehydrogenase kinase, isozyme 1 | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1(408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PDK1 |
Protein |
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial |
Uniprot ID |
Q15118 |
Function |
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress. |
Tissue Specificity |
Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas. |
Sub-cellular localization |
Mitochondrion matrix. |
Sequence Similarities |
Belongs to the PDK/BCKDK protein kinase family. |
Aliases |
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 antibody|[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody|HGNC:8809 antibody|mitochondrial antibody|Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody|PDH kinase 1 antibody|Pdk1 antibody|PDK1_HUMAN antibody|Pyruvate dehydrogenase kinase isoform 1 antibody|Pyruvate dehydrogenase kinase, isoenzyme 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, ASA-B1279, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: M231 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: SW620 Cell Lysate
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: M231 Cell Lysate
Lane 5: COLO320 Cell Lysate
Lane 6: SW620 Cell Lysate
Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody, ASA-B1279, IHC(P)
IHC(P): Human Prostatic Cancer Tissue
IHC(P): Human Prostatic Cancer Tissue
