Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| matrix metallopeptidase 12(macrophage elastase) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MMP12(428-444aa KIDAVFYSKNKYYYFFQ). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MMP12 |
Protein |
Macrophage metalloelastase |
Uniprot ID |
P39900 |
Function |
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1′ site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. |
Tissue Specificity |
Found in alveolar macrophages but not in peripheral blood monocytes. |
Sub-cellular localization |
Secreted, extracellular space, extracellular matrix . |
Sequence Similarities |
Belongs to the peptidase M10A family. |
Aliases |
EC 3.4.24.65 antibody|HME antibody|Macrophage elastase antibody|Macrophage metalloelastase antibody|Macrophage metaloelastase antibody|Matrix metallopeptidase 12(macrophage elastase) antibody|Matrix metalloprotease 12 antibody|Matrix metalloproteinase-12 antibody|ME antibody|MGC138506 antibody|MME antibody|MMP 12 antibody|MMP-12 antibody|Mmp12 antibody|MMP12_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MMP12 antibody, ASA-B1285, Western blotting
Lane 1: SMMC Cell Lysate
Lane 2: HEPA Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: K562 Cell Lysate
Lane 5: MCF-7 Cell Lysate
Lane 1: SMMC Cell Lysate
Lane 2: HEPA Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: K562 Cell Lysate
Lane 5: MCF-7 Cell Lysate
