Description
|
Assay Range |
0-600 ng/ml |
|
Sensitivity |
1.0 ng/mL |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Mouse Ceruloplasmin standard: 2 vials
2. One 96-well plate coated Mouse Ceruloplasmin Ab
3. Diluent buffer (5x): 12 ml
4.HRP conjugated Ab: 1 vial
5. TMB developing reagent : 12 ml.
6. Stop solution: 12 mL x1
7. Washing solution (20x): 25 mL x1
Background
Ceruloplasmin , also known as caeruloplasmin, is a multifunctional protein encoded by the CP gene in humans. Ceruloplasmin is the major copper-carrying protein in the blood, itcarries more than 95% of the total copper in healthy human plasma. It provides Cu2+ ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. In addition, it has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
