Description
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD105 standard: 5 ng/vial – 2.
2. One 96-well plate precoated with anti-Mouse CD105 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD105 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Endoglin (CD105) is a type I membrane glycoprotein located on cell surfaces and is part of the TGF beta receptor complex. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. It has been postulated that endoglin is involved in the cytoskeletal organization affecting cell morphology and migration. Endoglin has been regarded as a powerful angiogenic marker in endometrial carcinoma.
Data Sheet
| Assay Range | 31.25 – 2000 pg/ml |
