Description
Sensitivity
100 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse IFNAR1 standard: 10 ng/vial – 2.
2. One 96-well plate precoated with anti-Mouse IFNAR1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse IFNAR1 Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Interferon alpha/beta receptor 1, also known as IFN-alpha/beta receptor 1, is a single-pass type I membrane protein which belongs to thetype II cytokine receptor family. The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2.
Data Sheet
| Assay Range | 125 – 8000 pg/ml |
