Description
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse IL-13RA1 standard: 3 ng/vial – 2.
2. One 96-well plate precoated with anti-Mouse IL-13RA1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse IL-13RA1 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Interleukin 13 receptor, alpha 1, also known as IL13RA1 and CD213A1, is a subunit of the interleukin 13 receptor. IL13RA1 forms a receptor complex with IL4 receptor alpha (IL4RA), a subunit shared by IL13 and IL4 receptors. Interleukin 13 possesses two types of receptor: the heterodimer, composed of the IL13RA1 and the IL4RA, transducing the IL13 signals; and the IL13RA2, acting as a nonsignaling “decoy“receptor. IL13RA1 binds tyrosine kinase TYK2 and mediates the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.
Data Sheet
| Assay Range | 12 – 750 pg/ml |
