Description
Storage
Store at 4 °C.
Expiration
6 months at 4 °C.
Specificity
No detectable cross-reactivity with any other cytokine.
Application
cell culture supernatants, serum and plasma.
Kit Components
1. Lyophilized recombinant Mouse IL-5 standard: 50 pg/tube—2.
2. One 96-well plate precoated with anti-Mouse IL-5 antibody.
3. Sample diluent buffer: 12 mL —2
4. Biotinylated anti-Mouse IL-5 antibody: 60 µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 300 µL dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12ml.
8. Chromogen-A, 6ml; Chromogen-B, 6ml.
9. Stop Solution: 6ml.
Data Sheet
| Alternate Names | B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I |
| Assay Range | 0.78 – 50 pg/mL |
| Assay Time | 4.5 hours |
| Assay Type | Sandwich ELISA |
| Background | IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion – primarily IgA. It is also a key mediator in eosinophil activation. The IL-5 gene is located on chromosome 11 in the mouse, and chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF),which are often co-expressed in TH2 cells. Interleukin-5 is also expressed by eosinophils and has been observed in the mast cells of asthmatic airways by immunohistochemistry. IL-5 expression is regulated by several transcription factors including GATA3. |
| Gene ID | 16191 |
| Kit Type | Colorimetric ELISA |
| Sample | 5-10 µL mouse serum/plasma |
| Sample Type | Serum, Plasma, Cell supernatant |
| Sensitivity | 0.25 pg/mL |
| Size | 96T |
| UniProt ID | P04401 |
