Description
Mouse Interleukin 6 ELISA Kit
Different lot may vary, please follow the instruction coming with the kit.
Data Sheet
| Assay principle | The Mouse IL-6 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Mouse IL-6 specific antibody has been pre-coated onto 96-well plate. Mouse IL-6 present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Mouse IL-6 detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Mouse IL-6 captured on plate. |
| Assay Range | 62.5 – 4000 pg/mL |
| Assay time | 4.5 Hours |
| Background | IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. |
| Dilution Factor | Non-diluted |
| Sample Level | Non-detectable for healthy mouse |
| Sample type | Cell lysates, serum/ plasma and other suitable sample solution. |
| Sensitivity | 4 pg/mL |
| Specificity | No cross reactivity with relevant targets |
