Description
Data Sheet
| Alternative Name of Analyte | Ocam；RNCAM；Ncam-2 |
| Assay duration | 4 hours |
| Assay format | Sandwich |
| Assay principle | This Mouse OCAM ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse OCAM specific antibodies were precoated onto 96-well plates. The Mouse OCAM specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse OCAM amount of sample captured in plate. |
| Assay Range | 156.25 -10000 pg/ml |
| Background | Neural Cell Adhesion Molecule (NCAM, also the cluster of differentiation CD56) is a homophilic binding glycoprotein expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory. |
| Sample type | serum, plasma, cell culture supernatant |
| Sensitivity | 15 pg/ml |
| Size | 96 Tests |
| Specificity | reactive with Mouse OCAM, reactivity with other related proteins not observed |
| Storage | 2-8℃ |
