Description
Sensitivity
40 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD100 standard: 10 ng/vial – 2.
2. One 96-well plate precoated with anti-Mouse CD100 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD100 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Semaphorin 4D (Sema 4D) is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein (GAP) and repels axon growth cones in both the mature central nervous system.In the immune system, CD100 binds CD72 to activate B cells and dendritic cells, though much about this interaction is still under investigation. During skin damage repairs, SEMA4D interacts with Plexin B2 on gamma delta t cells to play a role in the healing process.
Data Sheet
| Assay Range | 47 – 3000 pg/ml |
