Description
Data Sheet
| Assay principle | The Mouse TSH ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Mouse TSH specific antibody has been pre-coated onto 96-well plate. Mouse TSH present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Mouse TSH detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Mouse TSH captured on plate. |
| Assay Range | 1.25 – 80 ng/ml |
| Assay time | 3.5 hours |
| Size | 96T |
