Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| N-methylpurine-DNA glycosylase | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human MPG(228-243aa NKSFDQRDLAQDEAVW), different from the related mouse sequence by two amino acids, and from related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MPG |
Protein |
DNA-3-methyladenine glycosylase |
Uniprot ID |
P29372 |
Function |
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Belongs to the DNA glycosylase MPG family. |
Aliases |
3 alkyladenine DNA glycosylase antibody|3-alkyladenine DNA glycosylase antibody|3-methyladenine DNA glycosidase antibody|3MG_HUMAN antibody|AAG antibody|ADPG antibody|Alkyladenine DNA glycosylase antibody|anpg antibody|APNG antibody|DNA 3 methyladenine glycosylase antibody|DNA-3-methyladenine glycosylase antibody|MDG antibody|Mpg antibody|N methylpurine DNA glycosirase antibody|N methylpurine DNA glycosylase antibody|N-methylpurine-DNA glycosylase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- MPG antibody, ASA-B1309, Western blotting
All lanes: Anti MPG (ASA-B1309) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti MPG (ASA-B1309) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
