Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cholinergic receptor, muscarinic 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of human Muscarinic Acetylcholine Receptor 2(356-370aa EKQNIVARKIVKMTK), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CHRM2 |
Protein |
Muscarinic acetylcholine receptor M2 |
Uniprot ID |
P08172 |
Function |
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Note: Phosphorylation in response to agonist binding promotes receptor internalization. |
Sequence Similarities |
|
Aliases |
7TM receptor antibody|Acetylcholine receptor, muscarinic, 2 antibody|Acm2 antibody|ACM2_HUMAN antibody|Cholinergic receptor muscarinic 2 antibody|Cholinergic receptor, muscarinic 2, cardiac antibody|Cholinergic receptor, muscarinic 2, isoform a antibody|Cholinergic receptor, muscarinic 2a antibody|CHRM 2 antibody|CHRM2 antibody|chrm2a antibody|CM2 antibody|FLJ43243 antibody|HM 2 antibody|HM2 antibody|M2 antibody|M2 muscarinic receptor antibody|MGC120006 antibody|MGC120007 antibody|Muscarinic acetylcholine receptor M2 antibody|Muscarinic M2 receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Muscarinic Acetylcholine Receptor 2 antibody, ASA-B1332, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
Anti-Muscarinic Acetylcholine Receptor 2 antibody, ASA-B1332,Western blotting
All lanes: Anti Muscarinic Acetylcholine Receptor 2 (ASA-B1332) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 52KD
All lanes: Anti Muscarinic Acetylcholine Receptor 2 (ASA-B1332) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 52KD
Observed bind size: 52KD
