Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| myeloid differentiation primary response 88 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human MyD88 recombinant protein (Position: A44-F264). Human MyD88 shares 84% and 83% amino acid (aa) sequences identity with mouse and rat MyD88, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
MYD88 |
Protein |
Myeloid differentiation primary response protein MyD88 |
Uniprot ID |
Q99836 |
Function |
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. |
Tissue Specificity |
Ubiquitous. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
Contains 1 death domain. |
Aliases |
MYD 88 antibody|Myd88 antibody|MYD88_HUMAN antibody|MYD88D antibody|Myeloid differentiation marker 88 antibody|Myeloid differentiation primary response gene 88 antibody|Myeloid differentiation primary response gene antibody|Myeloid differentiation primary response protein MyD88 antibody|OTTHUMP00000161718 antibody|OTTHUMP00000208595 antibody|OTTHUMP00000209058 antibody|OTTHUMP00000209059 antibody|OTTHUMP00000209060 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-MyD88 antibody, ASA-B1334–1.jpg
All lanes: Anti MYD88 (ASA-B1334) at 0.5ug/ml
WB: Recombinant Human MYD88 Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti MYD88 (ASA-B1334) at 0.5ug/ml
WB: Recombinant Human MYD88 Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
Anti-MyD88 antibody, ASA-B1334–2.jpg
All lanes: Anti MYD88 (ASA-B1334) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
All lanes: Anti MYD88 (ASA-B1334) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF Whole Cell Lysate at 40ug
Lane 4: HEPG2 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: RAJI Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
Anti-MyD88 antibody, ASA-B1334–3.jpg
IHC(P): Rat Lung Tissue
IHC(P): Rat Lung Tissue
