Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| myosin, heavy chain 1, skeletal muscle, adult; myosin, heavy chain 2, skeletal muscle, adult |
Monoclonal |
IgG |
Mouse |
Human, Mouse, Rabbit, Rat |
IHC-P, WB |
Ascites |
Immunogen
|
| Rabbit muscle myosin. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
MYH1; MYH2 |
Protein
|
Myosin-1; Myosin-2 |
Uniprot ID
|
Human(P12882), Mouse(Q5SX40), Rat(F1LRV9), Human(Q9UKX2), Mouse(I6L963) |
Function
|
Muscle contraction. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cytoplasm, myofibril. Note: Thick filaments of the myofibrils. |
Sequence Similarities
|
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. |
Aliases
|
MYHa|MYHSA1|MyHC-2x|MyHC-2X/D|MyHC-IIx/d|MYH2A|MYHSA2|MYHas8|MyHC-2A|MyHC-IIa|Myosin heavy chain 1|Myosin heavy chain 2x|Myosin heavy chain IIx/d|Myosin heavy chain, skeletal muscle, adult 1|Myosin heavy chain 2|Myosin heavy chain 2a|Myosin heavy chain IIa|Myosin heavy chain, skeletal muscle, adult 2 |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
1-2μg/ml |
Human, mouse, rabbit, rat |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
1.5-2μg/ml |
Human, mouse, rabbit, rat |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Myosin(Skeletal, Fast) antibody (monoclonal), ASA-B1342, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
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