Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| myosin, heavy chain 11, smooth muscle |
Monoclonal |
IgG |
Mouse |
Chicken, Human, Rabbit |
IHC-P, IHC-F, WB |
Ascites |
Immunogen
|
| Human uterus smooth muscle extract. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
MYH11 |
Protein
|
Myosin-11 |
Uniprot ID
|
Human(P35749), Mouse(O08638), Rat(Q63862) |
Function
|
Muscle contraction. |
Tissue Specificity
|
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles. |
Sub-cellular localization
|
Melanosome. |
Sequence Similarities
|
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. |
Aliases
|
AAT4|FAA4|SMHC|SMMHC|KIAA0866|Myosin heavy chain 11|Myosin heavy chain, smooth muscle isoform |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.25-0.5μg/ml |
Human, chicken, rabbit |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human, chicken, rabbit |
AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) |
0.5-1μg/ml |
Human, chicken, rabbit |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
