Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| neural cell adhesion molecule 1 |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the C-terminus of human NCAM(657-673aa DHVMLKSLDWNAEYEVY), identical to the related rat and mouse sequences. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
NCAM1 |
Protein
|
Neural cell adhesion molecule 1(N-CAM-1/NCAM-1) |
Uniprot ID
|
P13591 |
Function
|
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. |
Tissue Specificity
|
|
Sub-cellular localization
|
Isoform 1: Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities
|
Contains 2 fibronectin type-III domains. |
Aliases
|
antigen recognized by monoclonal antibody 5.1H11 antibody|CD56 antibody|MSK 39 antibody|MSK39 antibody|N-CAM-1 antibody|NCAM 1 antibody|NCAM antibody|NCAM C antibody|NCAM-1 antibody|NCAM1 antibody|NCAM1_HUMAN antibody|NCAMC antibody|Neural cell adhesion molecule 1 antibody|Neural cell adhesion molecule NCAM antibody|OTTHUMP00000235666 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human, Mouse, Rat |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Rat Human, Mouse |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NCAM antibody, ASA-B1350, Western blotting
WB: Rat Brain Tissue Lysate
