Description
Data Sheet
| Amino acid sequence | YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT. |
| Biological Activity | The ED50 as determined by the dose-dependent proliferation of C6 cells and is 3.6-5.4?g/ml. |
| Description | Neurotrophin-3 Human Recombinant produced in E.Coli is a non-glycosylated and non-covalently linked homodimer, containing 2×119 amino acid chains, having a total Mw of 27.2 kDa.The NT-3 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | Lyophilized from 0.02% TFA. |
| Protein Background | NT3 a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. |
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | 1.Title:Generation of Dopamine Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant[1]^[2]^.Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press.Link:http://onlinelibrary.wiley.com/doi/10.1002/stem.146/fullLinks:1.http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full#fn12.http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full#fn3 2.Title:Mash1 and Neurogenin 2 Enhance Survival and Differentiation of Neural Precursor Cells After Transplantation to Rat Brains via Distinct Modes of Action.Publication: Received 22 May 2008; Accepted 8 August 2008; Published online 9 September 2008.Molecular Therapy (2008) 16 11, 18731882 doi:10.1038/mt.2008.189Link:http://www.nature.com/mt/journal/v16/n11/full/mt2008189a.html3.Title: TrkA Receptor Hot Spots for Binding of NT-3 as a Heterologous Ligand Publications: Journal of Biological Chemistry 282.23 (2007): 16754-16763. Link: http://www.jbc.org/content/282/23/16754.full4.Title: Orphan nuclear receptor Nurr1 induces neuron differentiation from embryonic cortical precursor cells via an extrinsic paracrine mechanismPublication: FEBS letters 583.9 (2009): 1505-1510Link http://www.sciencedirect.com/science/article/pii/S0014579309002828 |
| Solubility | It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3. |
