Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| nitric oxide synthase 1(neuronal) |
Monoclonal |
IgG |
Mouse |
Goat, Human, Pig, Rat |
WB |
Ascites |
Immunogen
|
| Recombinant neuronal NOS fragment(amino acids 1-181) from rat brain. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
NOS1 |
Protein
|
Nitric oxide synthase, brain |
Uniprot ID
|
Human(P29475), Mouse(Q9Z0J4), Rat(P29476) |
Function
|
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. |
Tissue Specificity
|
Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland. |
Sub-cellular localization
|
Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine . Note: In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity). |
Sequence Similarities
|
Belongs to the NOS family. |
Aliases
|
NOS|bNOS|nNOS|IHPS1|N-NOS|NC-NOS|Neuronal NOS|Peptidyl-cysteine S-nitrosylase NOS1 |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.5μg/ml |
Human, goat, pig, rat |
AssaySolutio’s ECL kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
