Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| N-myc (and STAT) interactor | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human NMI recombinant protein (Position: E2-E307). Human NMI shares 64% amino acid (aa) sequence identity with mouse NMI. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NMI |
Protein |
N-myc-interactor |
Uniprot ID |
Q13287 |
Function |
May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. |
Tissue Specificity |
Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
N myc (and STAT) interactor antibody|N myc and STAT interactor antibody|N myc interactor antibody|NMI antibody|NMYC and STAT interactor antibody|NMYC interactor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- NMI antibody, ASA-B1394, Western blotting
All lanes: Anti NMI (ASA-B1394) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
All lanes: Anti NMI (ASA-B1394) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
Anti- NMI antibody, ASA-B1394, IHC(P)
IHC(P): Human Intestinal Tissue
IHC(P): Human Intestinal Tissue
