Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nephrosis 2, idiopathic, steroid-resistant(podocin) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of human NPHS2(368-383aa KPVEPLNPKKKDSPML), identical to the related mouse sequence, and different from the related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NPHS2 |
Protein |
Podocin |
Uniprot ID |
Q9NP85 |
Function |
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. |
Tissue Specificity |
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. |
Sub-cellular localization |
Isoform 1: Cell membrane ; Peripheral membrane protein . |
Sequence Similarities |
Belongs to the band 7/mec-2 family. |
Aliases |
Nephrosis 2, idiopathic, steroid resistant(podocin) antibody|nephrosis 2, idiopathic, steroid resistant antibody|NPHS2 antibody|NPHS2 gene antibody|PDCN antibody|PODO_HUMAN antibody|Podocin antibody|SRN1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Human, Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Rat Human, Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NPHS2 antibody, ASA-B1405, Western blotting
WB: Rat Kidney Tissue Lysate
WB: Rat Kidney Tissue Lysate
Anti-NPHS2 antibody, ASA-B1405, IHC(F)
IHC(F): Rat Kidney Tissue
IHC(F): Rat Kidney Tissue
