Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| nephrosis 2, idiopathic, steroid-resistant(podocin) |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence in the middle region of human NPHS2(126-143aa CVKVVQEYERVIIFRLGH), different from the mouse sequence by one amino acid. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
NPHS2 |
Protein
|
Podocin |
Uniprot ID
|
Q9NP85 |
Function
|
Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. |
Tissue Specificity
|
Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli. |
Sub-cellular localization
|
Isoform 1: Cell membrane ; Peripheral membrane protein . |
Sequence Similarities
|
Belongs to the band 7/mec-2 family. |
Aliases
|
Nephrosis 2, idiopathic, steroid resistant(podocin) antibody|nephrosis 2, idiopathic, steroid resistant antibody|NPHS2 antibody|NPHS2 gene antibody|PDCN antibody|PODO_HUMAN antibody|Podocin antibody|SRN1 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Rat Human, Mouse |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NPHS2 antibody, ASA-B1406, Western blotting
WB: Rat Kidney Tissue Lysate
