Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| neuregulin 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of human NRG2(289-306aa NGRKNSRLQFNKVKVEDA), identical to the related rat sequence, and different from the related mouse sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NRG2 |
Protein |
Pro-neuregulin-2, membrane-bound isoform(Pro-NRG2) |
Uniprot ID |
O14511 |
Function |
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. May also promote the heterodimerization with the EGF receptor. |
Tissue Specificity |
Restricted to the cerebellum in the adult. |
Sub-cellular localization |
Pro-neuregulin-2, membrane-bound isoform: Cell membrane ; Single-pass type I membrane protein . Note: Does not seem to be active. |
Sequence Similarities |
Belongs to the neuregulin family. |
Aliases |
Divergent of neuregulin 1 antibody|Divergent of neuregulin-1 antibody|Don 1 antibody|DON-1 antibody|Neural- and thymus-derived activator for ERBB kinases antibody|Neuregulin 2 antibody|Neuregulin-2 antibody|NRG-2 antibody|Nrg2 antibody|NRG2_HUMAN antibody|NTAK antibody|Pro NRG2 antibody|Pro-NRG2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NRG2 antibody, ASA-B1417, Western blotting
All lanes: Anti NRG2 (ASA-B1417) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
All lanes: Anti NRG2 (ASA-B1417) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 91KD
Observed bind size: 91KD
