Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| nuclear receptor subfamily 4, group A, member 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human NUR77(99-112aa ASFKFEDFQVYGCY), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
NR4A1 |
Protein |
Nuclear receptor subfamily 4 group A member 1 |
Uniprot ID |
P22736 |
Function |
Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5′- AAAAGGTCA-3′ (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. |
Tissue Specificity |
Fetal muscle and adult liver, brain and thyroid. |
Sub-cellular localization |
Cytoplasm. Nucleus. |
Sequence Similarities |
Belongs to the nuclear hormone receptor family. NR4 subfamily. |
Aliases |
Early response protein NAK1 antibody|GFRP 1 antibody|GFRP antibody|GFRP1 antibody|Growth factor inducible nuclear protein N10 antibody|Growth Factor Inducible Nuclear Protein NP10 antibody|Growth Factor Response Protein 1 antibody|Hbr1 antibody|HMR antibody|Hormone Receptor antibody|MGC9485 antibody|N10 antibody|N10 nuclear protein antibody|NAK 1 antibody|NAK1 antibody|Nerve growth factor IB nuclear receptor variant 1 antibody|NGFIB antibody|NP 10 antibody|NP10 antibody|NR4A1 antibody|NR4A1_HUMAN antibody|Nuclear hormone receptor NUR/77 antibody|Nuclear Hormone Receptor TR3 antibody|Nuclear Receptor Subfamily 4 Group A Member 1 antibody|NUR 77 antibody|NUR77 antibody|Orphan nuclear receptor HMR antibody|Orphan nuclear receptor NR4A1 antibody|Orphan nuclear receptor TR3 antibody|ST 59 antibody|ST-59 antibody|ST59 antibody|Steroid receptor TR3 antibody|Testicular receptor 3 antibody|TR 3 antibody|TR3 antibody|TR3 orphan receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-NUR77 antibody, ASA-B1426, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
